By using a ploidy-aware genotype calling based on likelihoods, we generated a dataset of 16,107 high-quality SNPs. In addition, we included two populations of A. tinctoria within its main distribution range in Greece and genotyped them using restriction site-associated DNA sequencing.
Methods: We collected 14 populations of A. From a practical perspective, such investigation can inform biodiversity management strategies. Here, by employing a ploidy-aware framework, we investigated the genetic structure and its determinants in polyploid Alkanna tinctoria (Boraginaceae), an ancient medicinal herb that is the source of bioactive compounds known as alkannin and shikonin (A/S). In comparison with diploids, however, genetic variation and factors fostering genetic divergence remain poorly studied in polyploids due to analytical challenges. 3Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, Thessaloniki, Greeceīackground and Aims: Quantifying genetic variation is fundamental to understand a species’ demographic trajectory and its ability to adapt to future changes.2Department of Botany and Biodiversity Research, University of Vienna, Vienna, Austria.1Center for Health & Bioresources, AIT Austrian Institute of Technology GmbH, Tulln, Austria.Temsch 2, Hanna Weiss-Schneeweiss 2, Christian Lexer 2 †, Eva Maria Sehr 1 * ‡ and Ovidiu Paun 2 ‡ Muhammad Ahmad 1,2, Thibault Leroy 2, Nikos Krigas 3, Eva M.
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